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Immune repertoire11/10/2023 By analysing human and mouse BCR repertoires at an unprecedented scale, we observed species-specific structural predetermination and detected CDR dynamics across multiple stages of B-cell differentiation. Here we report our novel rapid pipeline, SAAB+, which has enabled us to interrogate how the structure of the CDR changes in BCR repertoires along the B-cell differentiation axis. Drug discovery and immunodiagnostics inspired by the adaptive immune system rely on our ability to accurately interrogate the structural diversity of the binding sites of the BCR repertoire. Each individual has a huge BCR repertoire, where each individual BCR has a specific binding site composed of the complementary-determining regions (CDRs) capable of recognising a specific antigen. These are immunoglobulin molecules that bind to foreign substances known as antigens. ī-cell receptors (BCR) are the major components of the adaptive immune system. ![]() The software tool for structural annotation of BCR repertoires, SAAB+, is available at. Our results establish the CDR structure differences in BCR repertoires and have applications for many fields including immunodiagnostics, phage display library generation, and “humanness” assessment of BCR repertoires from transgenic animals. In contrast, more differentiated B-cells are more personalized in terms of CDR structure usage. Antigen-unexperienced BCR repertoires use the highest number and diversity of CDR structures and these patterns of naïve repertoire paratope usage are highly conserved across subjects. We show that B-cell types can be distinguished based solely on these structural properties. These structurally annotated CDRs provide unprecedented insights into both the structural predetermination and dynamics of the adaptive immune response. We have used novel rapid methods to structurally characterize the complementary-determining regions (CDRs) of more than 180 million human and mouse B-cell receptor (BCR) repertoire sequences. The specificity allows the Immuno-Seq technology to be applied to disease surveillance, antibody production, vaccine research, health examination and other related areas.Most current analysis tools for antibody next-generation sequencing data work with primary sequence descriptors, leaving accompanying structural information unharnessed. Our Immuno-Seq System is designed to assist researchers in the observation and analysis of both T and B cells with unprecedented specificity. Synbio Technologies provides our customers with a complete antibody repertoire sequencing system including immune repertoire sequencing, antibody CDR region sequencing, etc. Combined with the high-throughput sequencing technology, the Immuno-Seq provides a comprehensive evaluation of the immune system diversity. The CDRs are targeted because they control the diversity of the corresponding BCRs and TCRs. The 5’ RACE technology amplifies complementarity-determining regions (CDRs) of B cell receptors (BCRs) or T cell receptors (TCRs). Immune repertoire sequencing (Immuno-Seq) targets at T/B lymphocytes and utilizes the 5’ Rapid Amplification of cDNA Ends (RACE) Technology. ![]() Immune Repertoire refers to the set of all functionally diversified B cells and T cells in the circulatory system of an individual at any given time. ![]() Applications and Research of Gene Therapy.Target Screening for New Drug Development.Ultra-long Genome Synthesis and Assembly.Codon Optimization for Peptide and Protein Expression.Applications of Gene Synthesis on the Syno Platform.
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